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lung cancer cell lines hcc44  (DSMZ)


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    DSMZ lung cancer cell lines hcc44
    Circ-10720 regulates VIM and affects cell morphology in NSCLC cell lines. (A) Circ-10720 and CUL2 mRNA expression in the NSCLC cell lines A549, <t>HCC44,</t> H23 and H1299 in comparison to the expression in the BEAS2B normal immortalized lung cell line. (B) Heat map showing the expression of 29 EMT genes (data obtained from Cancer Cell Line Encyclopedia) in the four NSCLC cell lines studied. (C) Circ-10720 and CUL2 mRNA expression in HCC44 and A549 cells transfected with a siRNA against circ-10720 in comparison with control cells. (D) Representative Western blot analysis image of VIM and CDH1 after transfecting with circ-10720 siRNA. (E) Quantification of VIM and CDH1 relative protein levels in three independent replicates after silencing with circ-10720 siRNA. (F) Expression levels of miR-1246 in cells transfected with siRNA circ-10720 in comparison with control siRNA. (G) Immunofluorescence using VIM antibody (green) in control and circ-10720 silenced cells. (H) Bright field images of control or circ-10720 silenced cells. All bar plot data represented are the average of at least three independent replicates and error bars represent SEM. *, P<0.05; **, P<0.01. NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; VIM, Vimentin; CDH1, E-cadherin.
    Lung Cancer Cell Lines Hcc44, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lung cancer cell lines hcc44/product/DSMZ
    Average 94 stars, based on 55 article reviews
    lung cancer cell lines hcc44 - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Role of the epithelial-mesenchymal transition-related circular RNA, circ-10720, in non-small-cell lung cancer"

    Article Title: Role of the epithelial-mesenchymal transition-related circular RNA, circ-10720, in non-small-cell lung cancer

    Journal: Translational Lung Cancer Research

    doi: 10.21037/tlcr-20-920

    Circ-10720 regulates VIM and affects cell morphology in NSCLC cell lines. (A) Circ-10720 and CUL2 mRNA expression in the NSCLC cell lines A549, HCC44, H23 and H1299 in comparison to the expression in the BEAS2B normal immortalized lung cell line. (B) Heat map showing the expression of 29 EMT genes (data obtained from Cancer Cell Line Encyclopedia) in the four NSCLC cell lines studied. (C) Circ-10720 and CUL2 mRNA expression in HCC44 and A549 cells transfected with a siRNA against circ-10720 in comparison with control cells. (D) Representative Western blot analysis image of VIM and CDH1 after transfecting with circ-10720 siRNA. (E) Quantification of VIM and CDH1 relative protein levels in three independent replicates after silencing with circ-10720 siRNA. (F) Expression levels of miR-1246 in cells transfected with siRNA circ-10720 in comparison with control siRNA. (G) Immunofluorescence using VIM antibody (green) in control and circ-10720 silenced cells. (H) Bright field images of control or circ-10720 silenced cells. All bar plot data represented are the average of at least three independent replicates and error bars represent SEM. *, P<0.05; **, P<0.01. NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; VIM, Vimentin; CDH1, E-cadherin.
    Figure Legend Snippet: Circ-10720 regulates VIM and affects cell morphology in NSCLC cell lines. (A) Circ-10720 and CUL2 mRNA expression in the NSCLC cell lines A549, HCC44, H23 and H1299 in comparison to the expression in the BEAS2B normal immortalized lung cell line. (B) Heat map showing the expression of 29 EMT genes (data obtained from Cancer Cell Line Encyclopedia) in the four NSCLC cell lines studied. (C) Circ-10720 and CUL2 mRNA expression in HCC44 and A549 cells transfected with a siRNA against circ-10720 in comparison with control cells. (D) Representative Western blot analysis image of VIM and CDH1 after transfecting with circ-10720 siRNA. (E) Quantification of VIM and CDH1 relative protein levels in three independent replicates after silencing with circ-10720 siRNA. (F) Expression levels of miR-1246 in cells transfected with siRNA circ-10720 in comparison with control siRNA. (G) Immunofluorescence using VIM antibody (green) in control and circ-10720 silenced cells. (H) Bright field images of control or circ-10720 silenced cells. All bar plot data represented are the average of at least three independent replicates and error bars represent SEM. *, P<0.05; **, P<0.01. NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; VIM, Vimentin; CDH1, E-cadherin.

    Techniques Used: Expressing, Comparison, Transfection, Control, Western Blot, Immunofluorescence

    Circ-10720 regulates migration and invasion in NSCLC cell lines. (A) Images of one of the 3 replicates of the results of the wound healing assay for study of migration at 0, 12 and 24 hours after transfection with circ-10720 siRNA or control siRNA. The percentage included in each picture represents the wound closure percentage from 0 h. (B) Quantification of wound closure rates at different time points: 0, 6, 12 and 24 h. (C) Representative image of the matrigel-based invasion assay for HCC44 and A549 cells transfected with circ-10720 siRNA or control siRNA. (D) Quantitative analysis of invasion by dissolving crystal violet stained cells in 10% acetic acid and colorimetric reading of OD at 560 nm. Data is presented as ratio of control. All data represented in bar plots are the average of at least three independent replicates and error bars represents SEM. *, P<0.05; **, P<0.01. NSCLC, non-small cell lung cancer.
    Figure Legend Snippet: Circ-10720 regulates migration and invasion in NSCLC cell lines. (A) Images of one of the 3 replicates of the results of the wound healing assay for study of migration at 0, 12 and 24 hours after transfection with circ-10720 siRNA or control siRNA. The percentage included in each picture represents the wound closure percentage from 0 h. (B) Quantification of wound closure rates at different time points: 0, 6, 12 and 24 h. (C) Representative image of the matrigel-based invasion assay for HCC44 and A549 cells transfected with circ-10720 siRNA or control siRNA. (D) Quantitative analysis of invasion by dissolving crystal violet stained cells in 10% acetic acid and colorimetric reading of OD at 560 nm. Data is presented as ratio of control. All data represented in bar plots are the average of at least three independent replicates and error bars represents SEM. *, P<0.05; **, P<0.01. NSCLC, non-small cell lung cancer.

    Techniques Used: Migration, Wound Healing Assay, Transfection, Control, Invasion Assay, Staining

    Circ-10720 regulates apoptosis and proliferation in NSCLC cell lines. (A) Apoptosis rate in HCC44 and A549 cells transfected with circ-10720 siRNA in comparison with control cells. (B) HCC44 and (C) A549 proliferation analysis by MTS (absorbance at 490 nm) at 0, 24, 48 and 72 hours. All data represented are the average of at least three independent replicates and error bars represents SEM. *, P<0.05.
    Figure Legend Snippet: Circ-10720 regulates apoptosis and proliferation in NSCLC cell lines. (A) Apoptosis rate in HCC44 and A549 cells transfected with circ-10720 siRNA in comparison with control cells. (B) HCC44 and (C) A549 proliferation analysis by MTS (absorbance at 490 nm) at 0, 24, 48 and 72 hours. All data represented are the average of at least three independent replicates and error bars represents SEM. *, P<0.05.

    Techniques Used: Transfection, Comparison, Control



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    lung cancer cell lines hcc44  (DSMZ)
    94
    DSMZ lung cancer cell lines hcc44
    Circ-10720 regulates VIM and affects cell morphology in NSCLC cell lines. (A) Circ-10720 and CUL2 mRNA expression in the NSCLC cell lines A549, <t>HCC44,</t> H23 and H1299 in comparison to the expression in the BEAS2B normal immortalized lung cell line. (B) Heat map showing the expression of 29 EMT genes (data obtained from Cancer Cell Line Encyclopedia) in the four NSCLC cell lines studied. (C) Circ-10720 and CUL2 mRNA expression in HCC44 and A549 cells transfected with a siRNA against circ-10720 in comparison with control cells. (D) Representative Western blot analysis image of VIM and CDH1 after transfecting with circ-10720 siRNA. (E) Quantification of VIM and CDH1 relative protein levels in three independent replicates after silencing with circ-10720 siRNA. (F) Expression levels of miR-1246 in cells transfected with siRNA circ-10720 in comparison with control siRNA. (G) Immunofluorescence using VIM antibody (green) in control and circ-10720 silenced cells. (H) Bright field images of control or circ-10720 silenced cells. All bar plot data represented are the average of at least three independent replicates and error bars represent SEM. *, P<0.05; **, P<0.01. NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; VIM, Vimentin; CDH1, E-cadherin.
    Lung Cancer Cell Lines Hcc44, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lung cancer cell lines hcc44/product/DSMZ
    Average 94 stars, based on 1 article reviews
    lung cancer cell lines hcc44 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    lung cancer cell line hcc44  (DSMZ)
    90
    DSMZ lung cancer cell line hcc44
    ( A ) Western blot against exosomal marker TSG101 from HCC44 and <t>H23</t> cell lines under normoxic or hypoxic conditions. ( B ) Nanoparticle tracking analysis (NTA) report of the average distribution of particle size in a representative exosomal sample derived from HCC44 cells; lincRNA-p21 is overexpressed under hypoxic conditions in both ( C ) cell lines and ( D ) EVs. siRNA transfection against lincRNA-p21 under hypoxic conditions reduced its expression in both ( E ) cell lines and ( F ) EVs.
    Lung Cancer Cell Line Hcc44, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lung cancer cell line hcc44/product/DSMZ
    Average 90 stars, based on 1 article reviews
    lung cancer cell line hcc44 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    human lung cancer cell line hcc44  (DSMZ)
    94
    DSMZ human lung cancer cell line hcc44
    Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and <t>HCC44)</t> and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.
    Human Lung Cancer Cell Line Hcc44, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung cancer cell line hcc44/product/DSMZ
    Average 94 stars, based on 1 article reviews
    human lung cancer cell line hcc44 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Circ-10720 regulates VIM and affects cell morphology in NSCLC cell lines. (A) Circ-10720 and CUL2 mRNA expression in the NSCLC cell lines A549, HCC44, H23 and H1299 in comparison to the expression in the BEAS2B normal immortalized lung cell line. (B) Heat map showing the expression of 29 EMT genes (data obtained from Cancer Cell Line Encyclopedia) in the four NSCLC cell lines studied. (C) Circ-10720 and CUL2 mRNA expression in HCC44 and A549 cells transfected with a siRNA against circ-10720 in comparison with control cells. (D) Representative Western blot analysis image of VIM and CDH1 after transfecting with circ-10720 siRNA. (E) Quantification of VIM and CDH1 relative protein levels in three independent replicates after silencing with circ-10720 siRNA. (F) Expression levels of miR-1246 in cells transfected with siRNA circ-10720 in comparison with control siRNA. (G) Immunofluorescence using VIM antibody (green) in control and circ-10720 silenced cells. (H) Bright field images of control or circ-10720 silenced cells. All bar plot data represented are the average of at least three independent replicates and error bars represent SEM. *, P<0.05; **, P<0.01. NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; VIM, Vimentin; CDH1, E-cadherin.

    Journal: Translational Lung Cancer Research

    Article Title: Role of the epithelial-mesenchymal transition-related circular RNA, circ-10720, in non-small-cell lung cancer

    doi: 10.21037/tlcr-20-920

    Figure Lengend Snippet: Circ-10720 regulates VIM and affects cell morphology in NSCLC cell lines. (A) Circ-10720 and CUL2 mRNA expression in the NSCLC cell lines A549, HCC44, H23 and H1299 in comparison to the expression in the BEAS2B normal immortalized lung cell line. (B) Heat map showing the expression of 29 EMT genes (data obtained from Cancer Cell Line Encyclopedia) in the four NSCLC cell lines studied. (C) Circ-10720 and CUL2 mRNA expression in HCC44 and A549 cells transfected with a siRNA against circ-10720 in comparison with control cells. (D) Representative Western blot analysis image of VIM and CDH1 after transfecting with circ-10720 siRNA. (E) Quantification of VIM and CDH1 relative protein levels in three independent replicates after silencing with circ-10720 siRNA. (F) Expression levels of miR-1246 in cells transfected with siRNA circ-10720 in comparison with control siRNA. (G) Immunofluorescence using VIM antibody (green) in control and circ-10720 silenced cells. (H) Bright field images of control or circ-10720 silenced cells. All bar plot data represented are the average of at least three independent replicates and error bars represent SEM. *, P<0.05; **, P<0.01. NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; VIM, Vimentin; CDH1, E-cadherin.

    Article Snippet: Cryopreserved samples of the lung cancer cell lines HCC44 (ACC 534, DSMZ, Branuschweig, Germany), A549 (ACC 107, DSMZ), H23 (CRL-5800, ATTC, Manassas, VA, USA) and H1299 (CRL-5803, ATCC); and the normal immortalized lung cell line BEAS2B (95102433, ECACC, Sigma-Aldrich) were received in our laboratory and passaged for less than 6 months.

    Techniques: Expressing, Comparison, Transfection, Control, Western Blot, Immunofluorescence

    Circ-10720 regulates migration and invasion in NSCLC cell lines. (A) Images of one of the 3 replicates of the results of the wound healing assay for study of migration at 0, 12 and 24 hours after transfection with circ-10720 siRNA or control siRNA. The percentage included in each picture represents the wound closure percentage from 0 h. (B) Quantification of wound closure rates at different time points: 0, 6, 12 and 24 h. (C) Representative image of the matrigel-based invasion assay for HCC44 and A549 cells transfected with circ-10720 siRNA or control siRNA. (D) Quantitative analysis of invasion by dissolving crystal violet stained cells in 10% acetic acid and colorimetric reading of OD at 560 nm. Data is presented as ratio of control. All data represented in bar plots are the average of at least three independent replicates and error bars represents SEM. *, P<0.05; **, P<0.01. NSCLC, non-small cell lung cancer.

    Journal: Translational Lung Cancer Research

    Article Title: Role of the epithelial-mesenchymal transition-related circular RNA, circ-10720, in non-small-cell lung cancer

    doi: 10.21037/tlcr-20-920

    Figure Lengend Snippet: Circ-10720 regulates migration and invasion in NSCLC cell lines. (A) Images of one of the 3 replicates of the results of the wound healing assay for study of migration at 0, 12 and 24 hours after transfection with circ-10720 siRNA or control siRNA. The percentage included in each picture represents the wound closure percentage from 0 h. (B) Quantification of wound closure rates at different time points: 0, 6, 12 and 24 h. (C) Representative image of the matrigel-based invasion assay for HCC44 and A549 cells transfected with circ-10720 siRNA or control siRNA. (D) Quantitative analysis of invasion by dissolving crystal violet stained cells in 10% acetic acid and colorimetric reading of OD at 560 nm. Data is presented as ratio of control. All data represented in bar plots are the average of at least three independent replicates and error bars represents SEM. *, P<0.05; **, P<0.01. NSCLC, non-small cell lung cancer.

    Article Snippet: Cryopreserved samples of the lung cancer cell lines HCC44 (ACC 534, DSMZ, Branuschweig, Germany), A549 (ACC 107, DSMZ), H23 (CRL-5800, ATTC, Manassas, VA, USA) and H1299 (CRL-5803, ATCC); and the normal immortalized lung cell line BEAS2B (95102433, ECACC, Sigma-Aldrich) were received in our laboratory and passaged for less than 6 months.

    Techniques: Migration, Wound Healing Assay, Transfection, Control, Invasion Assay, Staining

    Circ-10720 regulates apoptosis and proliferation in NSCLC cell lines. (A) Apoptosis rate in HCC44 and A549 cells transfected with circ-10720 siRNA in comparison with control cells. (B) HCC44 and (C) A549 proliferation analysis by MTS (absorbance at 490 nm) at 0, 24, 48 and 72 hours. All data represented are the average of at least three independent replicates and error bars represents SEM. *, P<0.05.

    Journal: Translational Lung Cancer Research

    Article Title: Role of the epithelial-mesenchymal transition-related circular RNA, circ-10720, in non-small-cell lung cancer

    doi: 10.21037/tlcr-20-920

    Figure Lengend Snippet: Circ-10720 regulates apoptosis and proliferation in NSCLC cell lines. (A) Apoptosis rate in HCC44 and A549 cells transfected with circ-10720 siRNA in comparison with control cells. (B) HCC44 and (C) A549 proliferation analysis by MTS (absorbance at 490 nm) at 0, 24, 48 and 72 hours. All data represented are the average of at least three independent replicates and error bars represents SEM. *, P<0.05.

    Article Snippet: Cryopreserved samples of the lung cancer cell lines HCC44 (ACC 534, DSMZ, Branuschweig, Germany), A549 (ACC 107, DSMZ), H23 (CRL-5800, ATTC, Manassas, VA, USA) and H1299 (CRL-5803, ATCC); and the normal immortalized lung cell line BEAS2B (95102433, ECACC, Sigma-Aldrich) were received in our laboratory and passaged for less than 6 months.

    Techniques: Transfection, Comparison, Control

    ( A ) Western blot against exosomal marker TSG101 from HCC44 and H23 cell lines under normoxic or hypoxic conditions. ( B ) Nanoparticle tracking analysis (NTA) report of the average distribution of particle size in a representative exosomal sample derived from HCC44 cells; lincRNA-p21 is overexpressed under hypoxic conditions in both ( C ) cell lines and ( D ) EVs. siRNA transfection against lincRNA-p21 under hypoxic conditions reduced its expression in both ( E ) cell lines and ( F ) EVs.

    Journal: Cancers

    Article Title: Extracellular Vesicle lincRNA-p21 Expression in Tumor-Draining Pulmonary Vein Defines Prognosis in NSCLC and Modulates Endothelial Cell Behavior

    doi: 10.3390/cancers12030734

    Figure Lengend Snippet: ( A ) Western blot against exosomal marker TSG101 from HCC44 and H23 cell lines under normoxic or hypoxic conditions. ( B ) Nanoparticle tracking analysis (NTA) report of the average distribution of particle size in a representative exosomal sample derived from HCC44 cells; lincRNA-p21 is overexpressed under hypoxic conditions in both ( C ) cell lines and ( D ) EVs. siRNA transfection against lincRNA-p21 under hypoxic conditions reduced its expression in both ( E ) cell lines and ( F ) EVs.

    Article Snippet: Cryopreserved samples of the lung cancer cell lines H23 and HCC44 (American Type Culture Collection and DSMZ, respectively) were received in our laboratory and passaged for less than six months.

    Techniques: Western Blot, Marker, Derivative Assay, Transfection, Expressing

    Functional in vitro assays to evaluate the modulation of angiogenesis and endothelial cell permeability by EV treatment. ( A – D ) Tube formation assay using human umbilical vein endothelial cells (HUVECs) treated with control EVs in H23 ( A ) and HCC44 ( C ) and lincRNA-p21-silenced EVs in H23 ( B ) and HCC44 ( D ). ( E ) Quantification of relative number of adherent cells in a cell adhesion assay using a monolayer of HUVECs treated for 12 h with control EVs or lincRNA-p21-silenced EVs. ( F , G ) A representative image of HCC44 cell adhesion assay. Blue staining using DAPI indicates nucleus and HCC44 cells were stained with cell tracker in red (20× magnification). ( H , I ) lincRNA-p21 silencing reduced the EV levels of miR-23a, miR-146b, miR-330, and miR-494 in both cell lines. ( J , K ) EV conditioning increases the expression of miR-23a, miR-146b, miR-330, and miR-494 in HUVECs. ( L , M ) EV conditioning increases the expression of lincRNA-p21, GLUT1 , PFKFB3 , and GAPDH in HUVECs. * p < 0.05; ** p < 0.01.

    Journal: Cancers

    Article Title: Extracellular Vesicle lincRNA-p21 Expression in Tumor-Draining Pulmonary Vein Defines Prognosis in NSCLC and Modulates Endothelial Cell Behavior

    doi: 10.3390/cancers12030734

    Figure Lengend Snippet: Functional in vitro assays to evaluate the modulation of angiogenesis and endothelial cell permeability by EV treatment. ( A – D ) Tube formation assay using human umbilical vein endothelial cells (HUVECs) treated with control EVs in H23 ( A ) and HCC44 ( C ) and lincRNA-p21-silenced EVs in H23 ( B ) and HCC44 ( D ). ( E ) Quantification of relative number of adherent cells in a cell adhesion assay using a monolayer of HUVECs treated for 12 h with control EVs or lincRNA-p21-silenced EVs. ( F , G ) A representative image of HCC44 cell adhesion assay. Blue staining using DAPI indicates nucleus and HCC44 cells were stained with cell tracker in red (20× magnification). ( H , I ) lincRNA-p21 silencing reduced the EV levels of miR-23a, miR-146b, miR-330, and miR-494 in both cell lines. ( J , K ) EV conditioning increases the expression of miR-23a, miR-146b, miR-330, and miR-494 in HUVECs. ( L , M ) EV conditioning increases the expression of lincRNA-p21, GLUT1 , PFKFB3 , and GAPDH in HUVECs. * p < 0.05; ** p < 0.01.

    Article Snippet: Cryopreserved samples of the lung cancer cell lines H23 and HCC44 (American Type Culture Collection and DSMZ, respectively) were received in our laboratory and passaged for less than six months.

    Techniques: Functional Assay, In Vitro, Permeability, Tube Formation Assay, Control, Cell Adhesion Assay, Staining, Expressing

    Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and HCC44) and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.

    Journal: Scientific reports

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.

    doi: 10.1038/srep19857

    Figure Lengend Snippet: Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and HCC44) and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.

    Article Snippet: Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.

    Techniques: Mutagenesis, Expressing, Transfection, Plasmid Preparation